tracheal overexpression of il-1β, irak-1 and traf-6 mrna in obese-asthmatic male wistar rats

objective(s): human and animal studies have shown a close relationship between obesity and asthma severity. here, we examined the effects of diet-induced obesity (dio) on the expression levels of il-1β, irak-1 and traf-6 mrna in male wistar rats tracheal after sensitization with ovalbumin (ova). materials and methods:twenty male wistar rats divided to four groups, included, control group with normal diet (c+nd), ova-sensitized group with normal diet (s+nd), control group with high-fat diet (c+hfd), and ova-sensitized group with high-fat diet (s+hfd). all animals fed for 8 weeks with standard pelts or high-fat diet, and then were sensitized and challenged with ova or saline for another 4 weeks with designed regimens. at the end of study, trachea isolated and examined for expression levels of il-1β, irak-1 and traf-6 mrna with rt-pcr method. results:diet-induced obesity groups developed increased weight, obesity indexes and lipid profiles (p<0.05 to p<0.001). the expression levels of il-1β mrna in ova-sensitization groups (s+nd and s+hfd) showed a significantly increased when compared with control group. also in s+hfd group, expression level of traf-6 mrna was higher than other groups (p<0.001). irak-1 expression level was high in s+hfd compared with control group.il-1β and traf-6 mrna correlated positively with obesity indexes. conclusion:the results showed that dio causes overexpression of il-1β, irak-1 and traf-6 mrna in an experimental model of asthma. our results suggested that in obese-asthmatic conditions locally production and activation of pro-inflammatory agents can be increased. these findings showed that possible mechanism for obesity-asthma relationships.